Signalling

Part:BBa_K750108:Design

Designed by: Sifan Wang,Yizhen Yan,Rong Fan,Muxin Yu   Group: iGEM12_XMU-China   (2012-09-21)

PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1044
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719
    Illegal BsaI.rc site found at 1779


Design Notes

We still need a cI protein expression system liagted before this part to make the part work as we designed.


Source

We built this part by using the biobricks from the dna distribution plates 2012.

References

[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5