Signalling
Part:BBa_K750108:Design
Designed by: Sifan Wang,Yizhen Yan,Rong Fan,Muxin Yu Group: iGEM12_XMU-China (2012-09-21)
PcIGLT-pBADGLT:GFP(lva) expression system controlled by cI protein or arabinose
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1104
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1044
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 719
Illegal BsaI.rc site found at 1779
Design Notes
We still need a cI protein expression system liagted before this part to make the part work as we designed.
Source
We built this part by using the biobricks from the dna distribution plates 2012.
References
[1]Tamsir, A., J.J. Tabor, and C.A. Voigt, Robust multicellular computing using genetically encoded NOR gates and chemical 'wires'. Nature, 2011. 469(7329): p. 212-5